The authors confirm that all data underlying the findings are fully available without restriction. Some previous studies have identified bacteria in semen as being a potential factor in male infertility. However, only few types of bacteria were taken into consideration while using PCR-based or culturing methods. Here we present an analysis approach using next-generation sequencing technology and bioinformatics analysis to investigate the associations between bacterial communities and semen quality. Ninety-six semen samples collected were examined for bacterial communities, measuring seven clinical criteria for semen quality semen volume, sperm concentration, motility, Kruger's strict morphology, antisperm antibody IgA , Atypical, and leukocytes.
Facts about semen
Facts about semen | The Pig Site
The significance and diagnostic value of semen analysis in chronic bacterial prostatitis has been extensively debated and remains controversial. To investigate the diagnostic relevance of semen culture in the bacteriological workup of prostatitis patients, we retrospectively analyzed a clinical database of symptomatic patients. All patients were routinely subjected to a four-glass test, followed by semen culture and analysis. The 'five-glass' test showed 3. The area under the ROC curve and Jouden's index were increased, whereas positive and negative likelihood ratios were lower than comparators, indicating that the 'five-glass' assay may be superior in confirming the negative outcome of both standard tests.
The presence of bacteria species in semen and sperm quality
To verify the prevalence of semen bacterial contamination and whether the contamination could decrease sperm quality. Spermiogram, semen culture, and sperm transmission electron microscopy TEM analysis were performed. TEM data were elaborated using a mathematical formula that calculates a fertility index FI —able to define patients as fertile or infertile—and the percentage of sperm apoptosis, immaturity and necrosis. We aligned the amino acid sequence of beta-tubulin with protein of the most frequent species isolated from semen.
This content is taken from our book, Managing Pig Health, the industry leading pig publication. Available now from 5mBooks. Sperm consists of a head and a tail.